1. Obtain your team plates. Observe your set of “+pGLO” plates under room light and with UV light. Record numbers of colonies and color of colonies. Fill in the table below.
** In our actual results, the -pGLO LB was accidentally plated on the plate labeled -pGLO LB/amp and the -pGLO LB/amp was plated on the plate labeled -pGLO LB.
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2. The transformed bacteria now glow green under UV light because they have GFP (Glowing Fluorescent Protein). They also have resistance to ampicillin.
3. The carpet of bacteria on the -pGLO LB plate indicates that there were hundreds of bacteria in the 100 uL which reproduced to create thousands.
4. Arabinose is a sugar that gives bacteria the ability to glow under UV light. The plates which did not have arabinose did not glow under UV light.
5. If the GFP gene is attached to a protein inside a cell, it can act as a microscope and allow the inside of a cell to be seen. Also, GFP is an indicator of activity inside the cell, especially protein activity. It can monitor gene expression.
6. Bacteria can be genetically engineered to mass produce a protein product such as insulin, which is very useful in the medical field.
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| +pGLO LB/amp/ara plate: bacteria which contain GFP |
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